Further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the top 100 upregulated genes in Fbn1C1041G/+ VSMCs showed enrichment of IL-17 signaling, TNF signaling, chemokine signaling, and cytokine-cytokine receptor pathways, suggesting inflammatory activation in VSMCs during TAA pathogenesis in MFS (Supplemental Data File 2 and Figure 3B). This evidence concerns the gene TNF and Marfan syndrome.