To examine the functional impact of KAT7 in CRC cells, two lentiviral shRNAs (shKAT7-1/shKAT7-2) specifically targeting distinct regions of human KAT7, along with a puromycin selection gene, were individually transfected into HCT116 and COLO320 cells, which exhibited the highest expression levels of KAT7 (Figure 1F). The gene discussed is KAT7; the disease is colorectal carcinoma.