In vitro experiments showed that miR-576-3p overexpression inhibited GC proliferation, migration, invasion, and glycolysis metrics such as glucose uptake rate, while the overexpression of Circ-DNMT1 counteracted the inhibitory effect of miR-576-3p, suggesting that Circ-DNMT1 targeting miR-576-3p promotes GC progression and glycolysis. This evidence concerns the gene DNMT1 and gastric cancer.