As with the CDAA model, gbCNP−/− mice had greater hepatic fibrosis, immune cell infiltration into the liver, evidence of hepatocellular damage (i.e. plasma AST and ALT concentrations), and portal vein area compared with WT littermates (Figs. 3A, B, E–H, and L and S3B); however, and as expected, steatosis and liver and spleen weight were not significantly different (Fig. 3C, D, and I–K). Here, GPT is linked to steatosis.