Western Blot as well as qRT-PCR experiments further confirmed the expression of DUX4 and known DUX4-target genes29 TRIM48, TRIM49, ZSCAN4, ZSCAN5a, ZSCAN5d and RFPL4A upon HSV-1, HCMV and KSHV-infection (Fig. 1D, E, S1D, S2A) in different experimental settings. This evidence concerns the gene ZSCAN5A and infection.