It was next important to determine the role of Unc119 in TCR signal transduction, and so, we formed immune conjugates in vitro using OTI CD8+ T cells and SIINFEKL-expressing EG.7 lymphoma cells and quantified the polarisation of total LCK and pLCK Y394 to the IS as represented schematically (Fig 2H). Here, LCK is linked to lymphoma.