On the other hand, these approaches accentuated the role of the MMP2 gene at the FTO locus in skeletal myotubes, given its consistency within the GnRH signaling pathway (Figure 4—figure supplement 3), which is in line with previous studies linking its expression with obesity (Derosa et al., 2008; Aksoyer Sezgin et al., 2022; Nonino et al., 2021). This evidence concerns the gene MMP2 and obesity due to melanocortin 4 receptor deficiency.