Although rapid and excessive synaptic degranulation could theoretically result in bystander killing, as could the release of predocked LGs, the bystander killing of cells that do not promote NK cell activation and are resistant, such as osteosarcoma cells used in the present study (Figure S2A), suggests a direct role of LG dispersion (as prevented by GCC2) in bystander killing. The gene discussed is GCC2; the disease is osteosarcoma.