Overall, moderate agreement (κ = 0.44, 95% CI: 0.11–0.77) was demonstrated between the presence of Myf6 lineage and high Pax3::Foxo1 expression, implicating an interaction between Myf6 lineage and Pax3::Foxo1 during the development of these FP RMS tumours. The gene discussed is MYF6; the disease is neoplasm.