Further analysis with Leiden clustering revealed 14 distinct clusters (Figure 1A), and subsequent annotations showed populations of B cells, CD4+ T cells, Tregs, CD8+ T cells, mixed lymphocytes (consisting of naive, B cell, and T cell marker genes), mixed T cells (consisting of naive, CD4+, and CD8+ T cell genes), natural killer cells (NK), tumor associated macrophages (TAMs), dendritic cells (DCs), mast cells, cancer associated fibroblasts (CAFs), and cancer cells (Figures 1B and 1C). This evidence concerns the gene CD8A and cancer.