GSDMD and GSDME expression was eliminated in clones screened from LET1 cells co-transfected with the LentiCRISPRv2 vector targeting GSDMD and GSDME genes (Figure 3A); H1N1 virus infection did not induce pyroptosis in GSDMD/E-deficient LET1 cells as the cells did not present with the morphologically swelling characteristics of pyroptosis (Figures 3B and 3C). This evidence concerns the gene GSDMD and swine influenza.