NES and obesity due to melanocortin 4 receptor deficiency: However, the weight gain of Nestin-Cre mice was similar to the one of PI3KγF/F mice (Figures 4B and 4C), and PI3KγNest mice showed marked protection from diet-induced obesity compared to all the control groups, including Nestin-CRE mice (Figures 4A and 4B).