KCNJ11 and Glucose intolerance: To validate this newly generated Kir6.2 KO mouse line, we conducted comparative analyses with the original Kir6.2 KO mouse line.41 Both models displayed comparable glucose-related phenotypes, including glucose intolerance (two-way ANOVA with Šídák’s correction, P < 0.0001, F (1, 23) = 72.00) (Figure 1D) and impaired GSIS without affecting insulin sensitivity (two-way ANOVA with Šídák’s correction, P = 0.0155, F (1, 23) = 6.840) nor body weight (two-way ANOVA with Šídák’s correction, P = 0.8395, F (1, 11) = 0.04302) (Figure 1E, Supplementary Figure S4A).