Herein, levels of NLRP3, Caspase‐1, and GSDMD N/GSDMD FL in lung tissues of ALI mice were significantly increased, which were remarkably repressed by 0.1 and 0.5 μg AnxA1, implying a repressive function of AnxA1 against NLRP3 activation during ALI (Figure 6A,B). This evidence concerns the gene ANXA1 and acute respiratory distress syndrome.