To confirm the role of AdipoR1/AMPK/SIRT1 signaling pathway in the improvement of HepG2 cell steatosis by YCLLT, we silenced AdipoR1 to further explore the molecular mechanism of YCLLT, and the results showed that silencing of AdipoR1 inhibited the improvement of YCLLT in NAFLD cell model. This evidence concerns the gene SIRT1 and steatosis.