For instance, a study showed that priming with a recombinant TB protein (rMT1721) combined with GLA adjuvant, followed by a plasmid DNA vaccine expressing the same antigen, induced a more robust immune response, including both antigen-specific CD4 + and CD8 + T cells, compared to the reverse order, where CD8 + T cells were undetectable76. This evidence concerns the gene CD4 and tuberculosis.