TF and cancer: While HCs had a median TF of 0.016 (range 0.003–0.032), TFs of cancer samples ranged between 0.026 and 0.73 (median = 0.31), with OVCA ascites samples generally displaying high TFs (median = 0.43) compared to plasma samples (median: 0.10) (Fig. 3A; Supplemental Table S3).