MSLN and cancer: Mesothelin (MSLN) is a cell surface protein overexpressed in several cancers, including CRC, and is associated with tumor aggressiveness and poor prognosis.44-46 Histopathologic staining reveals MSLN expression in 48%-61% of CRC cases.47,48 CAR-T cells targeting MSLN can exploit its high expression on CRC cells to induce a potent antitumor response.49 In a preclinical study, the significant antitumor activity of third-generation MSLN-CAR-T cells was evaluated.