To more definitively understand the role of microtubule acetylation on HIV-1 infection and in TRIM69-mediated HIV-1 restriction, we examined both HIV-1 infection and TRIM69-mediated restriction of HIV-1 in cells lacking αTAT1, the acetyltransferase responsible for microtubule acetylation (5, 7, 8, 10). The gene discussed is TRIM69; the disease is HIV-1 infection.