Compared to Treg.1, Treg.2 showed significantly up-regulated genes (TNFRSF4/OX40, TNFRSF18/GITR, TNFRSF9/4–1BB, TNFRSF1B/TNFR2 and LAIR2) associated with suppressive functions of tumor-infiltrating Tregs49 (Supplementary Fig. 3.7e, Supplementary Table S8). This evidence concerns the gene TNFRSF1B and neoplasm.