While several studies investigated the effects of Artemisia species on human colon cancer cells (Khlifi et al. 2013; Ali, Saeed, and Omear 2021; Jakovljević et al. 2023), our study uniquely focused on assessing the cytotoxic properties across a diverse range of colorectal cancer cells including the isogenic cell lines HCT116 and RKO, which are differentiated only by their p53 status. Here, TP53 is linked to malignant colon neoplasm.