The cytotoxic effect of the methanolic extract of Artemisia herba‐alba was evaluated across a panel of colon cancer cell lines, including Caco2, DLD1, SW620, HCT116+/+p53, HCT116−/−p53, RKO+/+p53, and RKO−/−p53, SW620, and SW480. Here, TP53 is linked to colonic neoplasm.