The difference in P/LP variant frequency in SPRED1 (Legius syndrome) in the UKBB (1:19,567) and MyCode cohorts (1:41,762) (Table 1)24 is not explained by read depth differences in SPRED1 in the two cohorts (MyCode: 31.2; UK Biobank: 17.7). Here, SPRED1 is linked to Legius syndrome.