We established three stable isogenic knockout clones (V6.1-KO) in UMUC3, a bladder cancer cell line with high TERT expression (DepMap transcripts per million [TPM]=6.78 (Figure 2a, Figure S7a–b) and two clones in A549, a lung cancer cell line with moderate TERT expression (TPM=3.63, Figure S8a). The gene discussed is TERT; the disease is urinary bladder carcinoma.