Here, authors performed the cell viability study using developed cationized HSANPs loaded with EZH2siRNA (HSANPs‐PEI@EZH2siRNA) and free EZH2‐siRNA in both U937 and HL60 AML cells which revealed the IC50 values 29 ± 2.5 and 50 ± 10.11 nM in HL60 cells and 39 ± 15 nM and 60 ± 20 nM in U937 cells in 48 h, respectively. This evidence concerns the gene EZH2 and acute myeloid leukemia.