Of the 97 individuals who expressed interest in study participation and were screened for study eligibility, 55 did not meet inclusion criteria (including, as the top reasons for ineligibility: 13 were taking insulin, 11 had prior weight loss surgery or were taking weight loss medications, 8 had normal liver function tests, 6 had cirrhosis of the liver, 3 had heart failure or another related heart condition), 12 were eligible based on the prescreening survey but did not reply to follow-up requests, and 5 were eligible but declined to participate. Here, INS is linked to heart failure.