It has been demonstrated that TNIP3 negatively regulates NF-κB activation in response to LPS,32 and is also implicated in inflammatory bowel disease.12 We employed RNA-Seq of cardiac tissue from ISO-infused mice to explore the signalling networks related to ISO-induced cardiac dysregulation and TNIP3-mediated cardio-protection. The gene discussed is NFKB1; the disease is inflammatory bowel disease.