It was determined that 21 proteins (DHX9, EIF5, HPRT1, INPP5B, MCM6, PPP6C, SYF2, etc.)whose expression was increased in PC3‐R cells based on label‐free nLC‐MS/MS analysis were consistent with both TCGA prostate adenocarcinoma N0 and N1 nodal metastasis status and correlation data (Figure 8B). This evidence concerns the gene HPRT1 and prostate adenocarcinoma.