To explore which pathway/regulator was involved in METTL3 meditated ferroptosis in HCC, we used Western Blot analysis to detect the expression of major regulatory pathways of ferroptosis, including system xc--GPX4 (GPX4, SLC7A11), iron metabolism (CD71, Transferrin, FTH1, and HO-1) and lipid metabolism (ACSL4), results showed that only the expression of SLC7A11 is upregulated after IR treatment, and treatment of METTL3 knockdown group with IR did not increase the SLC7A11 expression. This evidence concerns the gene ACSL4 and hepatocellular carcinoma.