NLRP3 and neoplasm: To investigate the effects of myeloid cell NLRP3 activation on CTL-induced tumor rejection, PancOVA spheroids were cultured with combinations of macrophages (either treated or not treated with LPS and nigericin) and OVA-reactive OT-I WT or Il18r−/− CD8+ effector CTLs (Figure 4A,B).