ASNS and hepatocellular carcinoma: Analysis of metabolite profiling of 928 cell lines in the Cancer Cell Line Encyclopedia database indicated that lower asparagine levels elicited by the suppression of ASNS expression sensitize a subset of gastric cancer and hepatocellular carcinoma (HCC) cell lines, and that ASNS overmethylation results in a lack of ASNS protein expression, making them susceptible to ASNase both in vitro and in vivo [17].