Ye et al. reported that ATF4 knockdown decreased CRC cell survival and proliferation, and that overexpression of ASNS or asparagine supplementation reversed the growth inhibition and increased survival of ATF4-knockdown cells, which suggests that the GCN2-eIF2α-ATF4 pathway is important for metabolic homeostasis [59]. This evidence concerns the gene ASNS and colorectal carcinoma.