In this study, we found, for the first time, that interfering with THBS1 affected IVA replication in MDCK cells, and studies on the effect of THBS1 on influenza virus replication have not yet been reported, so THBS1 may be a potential target gene affecting influenza virus replication, showing potential promise for the study of potential influenza vaccines for the matrix of MDCK cells. This evidence concerns the gene THBS1 and influenza.