ACTA2 and Stroke: qPCR of RiboTag-IP’dmRNA from isolated microvessels after stroke revealed enrichment of astrocyte markers Aqp4 and Gfap [31,32], as well as depletion of vascular markers including Pecam1, Cldn5 (endothelial cells), Pdgfrb (pericytes), and Acta2 (vascular smooth muscle cells) (Figure 1F).