As described in Figure 5A, in the presence of TH301, there is a significant induction of the LC3B-II protein isoform (and the LC3B-I) in all three pancreatic cancer cell lines examined herein, with a simultaneous elevation of p62/SQSTM1 protein contents also detected, albeit in a time- and cell type-dependent manner (Figure 5A,B), which may indicate either induction of autophagy or impairment of autophagic flux. Here, SQSTM1 is linked to familial pancreatic carcinoma.