These findings strongly suggest that TH301 can induce G1-arrest by severely altering the expression patterns of cell cycle regulatory proteins (i.e., p21 induction in AsPC-1 cells {40 μM TH301}; Figure 2B,C) in all three pancreatic cancer cell lines, albeit following mutational signature-specific profiles. Here, PROS1 is linked to familial pancreatic carcinoma.