Additionally, the combination diet of ethanol and AD resulted in increased co-localization of TH (a sympathetic activation marker, purple, emission maximum = 670 nm) and α-SMA (an activated cardiac myofibroblast marker, green, emission maximum = 519 nm), appearing as pink, as well as increased co-localization of α-SMA and TGF-β1 (associated with fibrotic diseases, red, emission maximum = 570 nm), appearing as yellow. This evidence concerns the gene ACTA1 and Alzheimer disease.