Collecting liver and heart tissues at defined time points (e.g., 4, 8, and 12 weeks) for histological and molecular analysis to track inflammatory and fibrotic markers such as TGF-β1, α-SMA, and collagen deposition could be used to elucidate the temporal relationship between hepatic inflammation and cardiac fibrosis in a mouse model with MASH and LV MIF. The gene discussed is MIF; the disease is inflammatory response.