Our strategy allowed for the generation of several isogenic lines for IRD modeling, including the heritable p.V9M mutation, which is causative of NMNAT1-associated Leber congenital amaurosis, and the p.T494M mutation in PRPF3 and the p.H2309P mutation in PRPF8, both associated with retinitis pigmentosa. Here, PRPF8 is linked to retinitis pigmentosa.