Increased levels of hepatic cholesterol and triglycerides, elevated serum alanine transaminase and aspartate transaminase, and higher hepatic malondialdehyde levels in rats fed an HFD are indicative of hepatic steatosis and non-alcoholic steatohepatitis (NASH), consistent with our previous findings in this rat model [17]. The gene discussed is GPT; the disease is fatty liver disease.