Moreover, wound healing and Boyden chamber assays indicate that PRT543 significantly inhibits the migration (Fig. 3B and C) and invasion (Fig. 3D and E) abilities of both ACC cell lines compared to the untreated cells grown in parallel, supporting the role of PRMT5 in regulating the ACC tumorigenic potential in vitro. Here, PRMT5 is linked to adrenal cortex carcinoma.