The levels of PHKA2 mRNA and protein expression within the cell lines are presented in Supplementary Fig. S4G, H. PKM2 and ACLY expression was reduced significantly in PHKA2-knockdown cells and suppressed glycolipid metabolism and GBM cell growth dramatically (Supplementary Fig. S4I; Fig. 4C–J). This evidence concerns the gene PKM and glioblastoma.