BRCA2 and neoplasm: Furthermore, 2.9% (2/68) of the TRs obtained from the MASTER 1 analysis in comparison 1 were based on BMs that were not called in the panel (Fig. 3a): In addition to high HRD scores, a recommendation of PARP inhibition was based on a frameshift insertion at the 3’ end of BRCA2 with a tumor variant frequency (TVF) of 0.2, which was detected with a TVF of 0.049 in the panel sequencing and thus was filtered out due to a TVF threshold cutoff of 0.05 (patient 10; Supplementary Data 4–6).