To evaluate whether age, sex, and genetic variations correspond to specific RNA expression patterns in ALS, we re-run the analysis using age, sex, and the GGGGCC repeat size of C9orf72 or CAG repeat size of ATXN2 as the Machine-learning “labels” according to the clinical information of ALS samples. This evidence concerns the gene C9orf72 and amyotrophic lateral sclerosis.