In colony formation experiments, the same concentration of lenvatinib was added to each experimental group, and the colony formation rate of Huh-7 and HCC-LM3 cells with USP15 knocked down was significantly lower than that of corresponding wild-type HCC cells, DMSO was added to cells with USP15 knocked down as a control group. The gene discussed is USP15; the disease is hepatocellular carcinoma.