By contrast, all three LLGL2 tumours with somatic loss or promoter silencing of the wildtype allele showed absent or weaker, patchier staining for the protein (Supplementary Fig. 3b), indicating loss of LLGL2 protein synthesis in at least a portion of tumour cells in these samples, with some residual non-specific background staining in stroma and other cells present. The gene discussed is LLGL2; the disease is neoplasm.