Further validation through Western blot and immunofluorescence analyses demonstrated that Poly(I:C) treatment increased expression of TLR3, p-IRF3, and IFN-β in tumor tissues in the Poly group compared to the no-Poly group, confirming successful activation of the TLR3-IRF3-IFN-β pathway (Figures 8J, K). The gene discussed is IFNB1; the disease is neoplasm.