ProBDNF expression intensifies with glioma malignancy, predominantly in high‐grade tumors. Moreover, p75NTR expression parallels proBDNF, showing increased levels in high‐grade gliomas. Correlation analysis indicates positive associations between proBDNF, p75NTR, and malignancy grade. The proBDNF to mature BDNF ratio decreases with increasing tumor grade, suggesting a potential role in glioma progression. ProBDNF treatment induces morphological changes and differentiation in C6 glioma cells, potentially mediated through p75NTR activation. The gene discussed is BDNF; the disease is central nervous system cancer.