BDNF is significantly upregulated in glioma tissues and cells, correlating with higher clinical grading in glioma patients. Silencing BDNF inhibits glioma cell proliferation, migration, and invasion while promoting apoptosis. Furthermore, miR‐489‐3p negatively regulates BDNF expression through direct interaction, with reduced miR‐489‐3p levels observed in glioma tissues and cells, indicating its potential as a therapeutic target for glioma. The gene discussed is BDNF; the disease is central nervous system cancer.