Importantly, OTUD6B depletion could reduce KIFC1 expression in other cancer cell lines: MDA-MB-231, another TNBC cell line with moderate centrosome amplification, U2OS osteosarcoma cells that were originally used to demonstrate KIFC1 ubiquitination by the APC/C (Min et al, 2014), as well as in hTERT-HME1 as a model of immortalised normal breast epithelium (Fig. EV2E–G). Here, OTUD6B is linked to osteosarcoma.