IFNG and neoplasm: Because the tumor burden in the trimer group was too small to obtain sufficient amounts of T cells for investigations on antitumoral epitope-specific CD8+ T cells, we analyzed tumor-directed Adpgk-R304M-specific CD8+ T cells in splenocytes isolated 7 days after virotherapy and two applications of αPD1 by intracellular IFNγ staining (Fig. 7D).