Each experimental animal was followed for 5 days per week for 30 days.Our findings revealed that GLU-induced dermatitis via the upregulation of TNF-α, IL-1b, NFkb 1, and ptgs2 mRNA expression and the downregulation of TGFB1 mRNA expression caused oxidative stress and altered the biochemical markers and histological appearance. This evidence concerns the gene TNF and skin disorder.