Spearman’s correlation analysis demonstrated a significant negative correlation between Muribaculaceae, Eubacterium_ siraeum_ group, Lachnospiraceae_NK4A136_group and CKD-related biochemical indicators, including BUN, 24H/P, key proteins of the TLR4 pathway and LPS, indicators of fibrosis and inflammatory factors. The gene discussed is TLR4; the disease is chronic kidney disease.