But our research is unique as it is the first study focusing on NOS3 (rs1799983, rs2070744), PON1 (rs662), LPA-PLA2 (rs105193, rs1805017), APOE (rs429358, rs7412), PCSK9 (rs505151), MEF2A (rs325400), and LDLR (rs1122608, rs2228671) to investigate different genetic variants, pattern of inheritance and their relation with CAD specifically in families in Pakistan. This evidence concerns the gene NOS3 and coronary artery disorder.