TBG significantly inhibited the protein expression of PLK1 in HNSCC cells, thereby suppressing the expression of G2/M phase‐associated proteins including CDK1, CDC25c, and Cyclin B1, while the inhibited PLK1 was unable to promote nuclear ectasia of CTCF, thus inhibiting the metastasis of HNSCC cells. Here, CDK1 is linked to head and neck squamous cell carcinoma.